Tools in Biocatalysis Enzyme Immobilisation on Silica and Synthesis of Enantiopure Amines
نویسندگان
چکیده
This thesis presents two techniques in the field of biocatalysis: An enzyme immobilisation method based on the His6-tag for attachment on modified silica oxide beads, and it’s employment in aqueous and organic medium for synthesis applications. The method functions as a one step extraction and immobilisation protocol. An equilibrium displacement system which enables complete conversion in reactions with ω-transaminases where isopropylamine is the donor, a route for synthesis of pharmaceutically interesting enantiopure amines. Biocatalysis is predicted to be a paramount technology for an environmentally sustainable chemical industry, to which every newly developed method represents a small but important step. The work done here is aimed to be a part of this development.
منابع مشابه
Solid-binding peptides for immobilisation of thermostable enzymes to hydrolyse biomass polysaccharides
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